cd4 fitc cd8a r pe cd45 apc (SouthernBiotech)
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Cd4 Fitc Cd8a R Pe Cd45 Apc, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 147 article reviews
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Article Title: The effect of humic substances on gut microbiota and immune response of broilers
Journal: Food and Agricultural Immunology
doi: 10.1080/09540105.2019.1707780
Figure Legend Snippet: Figure 3. The effect of 0.8% of natural humic substances prepared from leonardite in feed on the per- centage of CD4+ CD8–, CD8+ CD4– and CD4+ CD8+ lymphocytes in broiler peripheral blood. Means with different superscripts are significantly different **p < .01.
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Clinical Proteomics:Article Title: The effect of humic substances on gut microbiota and immune response of broilers Article Snippet: For the lymphocyte phenotyping, mononuclear leukocytes (MNL) were isolated from heparinised blood as follows: 600 μl of blood was diluted in a 1:1 ratio with PBS (MP Biomedicals, France) and carefully underlayered with 2 ml of lymphocyte separating medium LSM 1077 (PAA Laboratories GmbH, Austria). .. Tubes were centrifuged for 30 min at 600× g. MNL were recovered by aspirating white layer in plasma-LSM interphase, and the acquired sample was washed twice with PBS by centrifugation for 5 min at 250× g. Selected lymphocyte subpopulations were identified by direct staining with a subsequent combination of conjugated anti-chicken monoclonal antibodies (MoAb): Centrifugation:Article Title: The effect of humic substances on gut microbiota and immune response of broilers Article Snippet: For the lymphocyte phenotyping, mononuclear leukocytes (MNL) were isolated from heparinised blood as follows: 600 μl of blood was diluted in a 1:1 ratio with PBS (MP Biomedicals, France) and carefully underlayered with 2 ml of lymphocyte separating medium LSM 1077 (PAA Laboratories GmbH, Austria). .. Tubes were centrifuged for 30 min at 600× g. MNL were recovered by aspirating white layer in plasma-LSM interphase, and the acquired sample was washed twice with PBS by centrifugation for 5 min at 250× g. Selected lymphocyte subpopulations were identified by direct staining with a subsequent combination of conjugated anti-chicken monoclonal antibodies (MoAb): Staining:Article Title: The effect of humic substances on gut microbiota and immune response of broilers Article Snippet: For the lymphocyte phenotyping, mononuclear leukocytes (MNL) were isolated from heparinised blood as follows: 600 μl of blood was diluted in a 1:1 ratio with PBS (MP Biomedicals, France) and carefully underlayered with 2 ml of lymphocyte separating medium LSM 1077 (PAA Laboratories GmbH, Austria). .. Tubes were centrifuged for 30 min at 600× g. MNL were recovered by aspirating white layer in plasma-LSM interphase, and the acquired sample was washed twice with PBS by centrifugation for 5 min at 250× g. Selected lymphocyte subpopulations were identified by direct staining with a subsequent combination of conjugated anti-chicken monoclonal antibodies (MoAb): Bioprocessing:Article Title: The effect of humic substances on gut microbiota and immune response of broilers Article Snippet: For the lymphocyte phenotyping, mononuclear leukocytes (MNL) were isolated from heparinised blood as follows: 600 μl of blood was diluted in a 1:1 ratio with PBS (MP Biomedicals, France) and carefully underlayered with 2 ml of lymphocyte separating medium LSM 1077 (PAA Laboratories GmbH, Austria). .. Tubes were centrifuged for 30 min at 600× g. MNL were recovered by aspirating white layer in plasma-LSM interphase, and the acquired sample was washed twice with PBS by centrifugation for 5 min at 250× g. Selected lymphocyte subpopulations were identified by direct staining with a subsequent combination of conjugated anti-chicken monoclonal antibodies (MoAb): |